Immunoperoxidase techniques and controls.

Immunoperoxidase technique in histopathology: applications, methods, and controls.

Assessment of virus infectivity by the immunofluorescent and immunoperoxidase techniques.

The immunofluorescent and immunoperoxidase cell-counting techniques were comparable in precision and reproducibility for the quantitative assessment of influenza virus infectivity. The dose-response function was linear with each procedure, and comparable results were obtained for estimating neutralizing antibodies in antiviral serum.

Comparison of techniques and immunoreagents used for indirect immunofluorescence and immunoperoxidase identification of enteroviruses.

Peroxidase-conjugated antibodies were found to be as sensitive as those conjugated to fluorescein-isothiocyanate (FITC) for identification of selected enterovirus types by the indirect technique. Peroxidase conjugates, however, were found to give fewer nonspecific reactions. The reasons for the higher specificity of the immunoperoxidase technique appear to be related to the relative size, charg...

Combination of immunofluorescence and immunoperoxidase techniques for serotyping mixtures of Mycoplasma species.

We describe a method for the simultaneous identification of up to three Mycoplasma species by the use of contrast-labeled fluorescent antibodies against two species and peroxidase-labeled antibody against a third species. The procedure enabled the rapid identification of colonies of three artificially mixed avian Mycoplasma species on agar blocks and also mixtures of species in cultures from na...

Immunoperoxidase staining of leptospires.

Leptospires were detectable by light microscopy when Formalin-fixed preparations were reacted by direct and indirect methods with horseradish peroxidase-labeled specific antibody.